化工儀器網(wǎng)
技術(shù)文章
BI－200SM研究級(jí)動(dòng)靜態(tài)光散射應(yīng)用案例-8
閱讀：545 發(fā)布時(shí)間：2014-10-23
文獻(xiàn)名： Porous-CLEAs of papain: Application to enzymatic hydrolysis of macromolecules
作者： Mengfan Wang, Chenxi Jia, Wei Qi, , Qingxin Yu, Xin Peng, Rongxin Su, Zhimin He
State Key Laboratory of Chemical Engineering, Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
摘要：Porous cross-linked enzyme aggregates (p-CLEAs) were prepared by adding starch as a pore-making agent, which facilitates CLEAs in cases where the substrates of enzyme are macromolecules. This novel strategy for preparation of p-CLEAs involves co-precipitation, cross-linking and removal of starch by α-amylase. The resulting papain p-CLEAs were characterized by scanning electron microscope (SEM) images, showing a porous structure. The 95.9% and 90.4% increased catalytic efficiencies of p-CLEAs than conventional CLEAs on bovine serum albumin (BSA) and ovalbumin verified the feasibility of this protocol.
關(guān)鍵詞： Enzymatic hydrolysis; Enzyme immobilization; Macromolecule; Papain; Porous cross-linked enzyme aggregates