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BioLife Solutions CS10 210102凍存液
閱讀：4709發(fā)布時(shí)間：2017-11-23
BioLife Solutions CryoStor CS10 細(xì)胞凍存液預(yù)混10% DMSO，適用于凍存多種類型細(xì)胞。
品牌：BioLife Solutions
貨號：PART No. 210102
容積：VOL. 100mL
型號：CryoStor CS10
BioLife Solutions CryoStor CS10細(xì)胞DMSO凍存液210102訂購信息
品牌 | 貨號 | 產(chǎn)品描述 | 規(guī)格 |
BioLife Solutions | 210102 | CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液 | 100mL /瓶 |
英文名：BioLife Solutions CryoStor CS10 Freeze Media
中文名：BioLife Solutions CryoStor CS10 細(xì)胞DMSO凍存液
BioLIfe Solutions CryoStor CS10細(xì)胞DMSO凍存液產(chǎn)品系列
各種規(guī)格的BioLIfe Solutions CryoStor 細(xì)胞DMSO凍存液
品牌 | 貨號 | 產(chǎn)品描述 | 規(guī)格 |
BioLife Solutions | 210102 | CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液 | 100mL /瓶 |
BioLife Solutions | 210210 | CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液 | 1000mL/袋 |
BioLife Solutions | 210202 | CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液 | 100mL/袋 |
BioLife Solutions | 210374 | CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液 | 16mL /瓶 |
BioLife Solutions | 210373 | CryoStor CS10 Freeze Media 細(xì)胞DMSO凍存液 | 10mL /瓶 |
BioLife Solutions CryoStor CS10細(xì)胞DMSO凍存液210102 代理銷售歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司 ：152 1681 4001。紅榮微再-客服: 316 808 6348 經(jīng)銷商專員
BioLife Solutions CryoStor CS10 Freeze Media 使用與凍存方法
CryoStor® Usage and Cryopreservation Protocol
細(xì)胞準(zhǔn)備
1) Place cells to be cryopreserved into suspension (mechanical or enzymatic dissociation)
2) Centrifuge cells to obtain cell pellet
3) Remove supernatant - Note: Remove as much culture media as possible, to reduce dilution of CryoStor® solution.
加CryoStor凍存液
4) ISOLATION: Add cold (2-8°C) CryoStor®
a. Cell concentrations: 0.5-10 x 106
cells/ml for routine cell culture protocols (higher [cell] possible).
b. DMSO is pre-mixed in CryoStor® - no additives are necessary.
5) PRE-FREEZE: Incubate cell suspension at 2-8°C for approximay 10 minutes
6) NUCLEATION: Freeze samples at -70°C (many protocols utlilize -70°C and -80°C interchangeably)
a. Use a controlled rate freeze (-1°C/min) or similar protocol for most mammalian cell systems.
b. The freezing device or isopropanol container should be pre-cooled to 2-8°C.
c. Ice nucleation within the sample (seeding) should be initiated at approximay -5°C using either a liquid nitrogen burst program setting on a controlled rate freezer or mechanical agitation (flick or tap) of the cryovial/sample container after approximay15-20 min. at -70°C.
d. Freeze time (-70°C) using isopropanol containers is recommended to be 3-4 hours.
BioLife Solutions 儲存
7) STORAGE: Place samples into storage
a. Store samples at liquid nitrogen temperatures (below -130°C).
b. Sample storage at -80°C is only recommended for short-term storage (weeks to months).
細(xì)胞復(fù)蘇解凍
8) THAWING: Thaw samples quickly in a 37°C water bath
a. Sample thawing should be conducted with gentle swirling of sample until all visible ice has melted. Approximate thaw time for a 1 ml sample in a cryovial is approximay 3 minutes.
b. DO NOT allow sample to warm above chilled temperatures (0-10°C). Cryovials should be cool to the touch when removed from bath.
Passive thaw is not recommended.
9) Dilute cell/CryoStor® mixture immediay with culture media
a. Dilution procedure can be preformed in a single step.
b. The dilution media should be between 20°C and 37°C.
c. A dilution ratio of 1:10 (sample to media) or greater is recommended.
10) Plate cells in appropriate configuration
11) Place cells into culture conditions or utilize immediay
12) Viability assessment 24-hours post-thaw*
BioLife Solutions Note: To obtain an accurate measure of cell viability following cryopreservation, assessment should be performed 24 hours post-thaw and compared to non-frozen controls.
*Sample assessment immediay post-thaw with membrane integrity indicators, such as Trypan Blue, for comparative analysis of sample cell yield and viability often results in significant overestimates of cell survival.
Live/Dead fluorescent assays or metabolic assays (MTT or alamarBlue®) are recommended for more accurate viability assessment.
Visual inspection of adherent cells and cells “floating” in the media is also recommended.
BioLife Solutions CryoStor CS10細(xì)胞DMSO凍存液210102 代理銷售歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司 ：152 1681 4001。紅榮微再-客服: 316 808 6348 經(jīng)銷商專員
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