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滬鼎產(chǎn)品文獻(xiàn):植物吲哚乙酸(IAA)ELISA試劑盒引用文獻(xiàn)
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滬鼎產(chǎn)品文獻(xiàn):植物吲哚乙酸(IAA)ELISA試劑盒引用文獻(xiàn)
【文獻(xiàn)標(biāo)題】Overexpression of the LcCUC2-likegene in Arabidopsis thaliana alters thecotyledon morphology and increases rosette leaf number
【作者】Shaoying Wen1,*, Jiayu Li1,*, Ziyuan Hao1,
【作者單位】南京林業(yè)大學(xué)
【DOI】 DOI 10.7717/peerj.12615
【影響因子(IF)】2.98
【文獻(xiàn)中引用產(chǎn)品】
植物吲哚乙酸(IAA)ELISA試劑盒
【出版期刊】《Overexpression of the LcCUC2-likegene in Arabidopsis thaliana alters thecotyledon morphology and increases rosette leaf number
【產(chǎn)品原文引用】Auxin content measurement
Basal rosette leaves samples of 0.6 g were harvested from 3-week-old WT and LcCUC2L
transgenic A. thaliana plants and immediately frozen in liquid nitrogen. Determination
of indole-3-acetic acid (IAA) in A. thaliana leaves was performed by ELISA (Huding,
Shanghai, China). Three replicates were used for each sample.
Histochemical staining to detect GUS activity
The β-Galactosidase Reporter Gene Staining Kit (Leagene) was used for GUS histochemical
staining in ProLcCUC2L transgenic and WT A. thaliana plants. In this study, seven-day-old
seedlings and fourteen-day-old seedlings were processed. Moreover, leaves at different
stages of development (10, 20 and 30 days of age) from plants planted in soil were
subjected to GUS staining. All materials were treated with GUS staining solution and
incubated overnight at 37 ?C, followed by bleaching with 75% (v/v) ethanol.
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【產(chǎn)品文獻(xiàn)支持】Auxin content measurement
Basal rosette leaves samples of 0.6 g were harvested from 3-week-old WT and LcCUC2L
transgenic A. thaliana plants and immediately frozen in liquid nitrogen. Determination
of indole-3-acetic acid (IAA) in A. thaliana leaves was performed by ELISA (Huding,
Shanghai, China). Three replicates were used for each sample.
影響因子(IF)= ：2.98 標(biāo)題:大學(xué):南京林業(yè)大學(xué)《Nanjing Forestry University》