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            研域（上海）化學(xué)試劑有限公司
                                                                        
            
										  中級(jí)會(huì)員 | 第18年									
            
                                    								
            

							
            
						
            
                        

					
            
										
            
						
            
                            							
            
								
            
								
            
									
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            實(shí)驗(yàn)細(xì)胞
            
											
            
											
											
            
																					
            
											
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            熱銷ELISA Kit
            
											
            
											
											
            
																					
            
											
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            Plant Phosphatidic acid ELISA kit
            
						
            
							時(shí)間：2010/4/19閱讀：1949
							
            
							
				
				分享：
				
				
						
            						
            
						
            
							 
             
            INTENDED USE 
            The PA ELISA is to be used for the quantitative determination of PA. The assay will recognize both natural and recombinant PA. The kit has been configured for research use only and is not to be used in diagnostic procedures.
             
            PRINCIPLE OF THE METHOD
            The PA kit is a solid phase phase sandwich enzyme linked immuno sorbent assay（ELISA）. Samples , including standards of known PA concentrations and unknowns are pipetted into these wells. During the first incubation, the PAantigen and a biotinylated monoclonal antibody specific for PAare simultaneously incubated. After washing, the enzyme（streptavidin-peroxydase）is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PApresent in the samples.
             
            REAGENTS PROVIDED AND RECONSTTTUTION
                        
                                    
            REAGENTS（Store at 2-8℃）
                                    		                        
            1×96 WELLS
                                    		                        
            0.5×96 WELLS
                                    		                        
            RECONSTTTUTION
                                    		        
                    
                                    
            96/48-wells microtiter plates
                                    		                        
            1
                                    		                        
            0.5
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Plastiv cover
                                    		                        
            2
                                    		                        
            1
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Standard: 40ng/ml
                                    		                        
            1Vials （0.6ml）
                                    		                        
            0.5Vials （0.3ml）
                                    		                        
            See reagents preparation on page 3
                                    		        
                    
                                    
            Blank control
                                    		                        
            1Vials （1.0ml）
                                    		                        
            1Vials  （0.5ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Standard Diluent
                                    		                        
            1Vials （5ml）
                                    		                        
            1Vials   （2.5ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Biotinylated anti-PA
                                    		                        
            1Vials （6ml）
                                    		                        
            1Vials   （3.0ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Streptavidin-HRP
                                    		                        
            1Vials （10ml）
                                    		                        
            1Vials   （5.0ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Washing Buffer
                                    		                        
            1Vials （60ml）
                                    		                        
            1Vials   （30ml）
                                    		                        
            20× concentrate
                                    		        
                    
                                    
            Substrate  A
                                    		                        
            1Vials （6.0ml）
                                    		                        
            1Vials   （3.0ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Substrate  B
                                    		                        
            1Vials 6.0ml）
                                    		                        
            1Vials   （3.0ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Stopping Solution
                                    		                        
            1Vials （6.0ml）
                                    		                        
            1Vials   （3.0ml）
                                    		                        
            Ready-to-use
                                    		        
                
             
            MATERIAL REQUIRED BUT NOT PROVIDED
            l       Distilled water
            l       Pipettes:10ul、50ul、100ul、200ul、1000ul。
            l       Vortex mixer and magnetic stirrer.
             
            INTENDED USE 
            The PA ELISA is to be used for the quantitative determination of PA. The assay will recognize both natural and recombinant PA. The kit has been configured for research use only and is not to be used in diagnostic procedures.
             
            PRINCIPLE OF THE METHOD
            The PA kit is a solid phase phase sandwich enzyme linked immuno sorbent assay（ELISA）. Samples , including standards of known PA concentrations and unknowns are pipetted into these wells. During the first incubation, the PAantigen and a biotinylated monoclonal antibody specific for PAare simultaneously incubated. After washing, the enzyme（streptavidin-peroxydase）is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PApresent in the samples.
             
            REAGENTS PROVIDED AND RECONSTTTUTION
                        
                                    
            REAGENTS（Store at 2-8℃）
                                    		                        
            1×96 WELLS
                                    		                        
            0.5×96 WELLS
                                    		                        
            RECONSTTTUTION
                                    		        
                    
                                    
            96/48-wells microtiter plates
                                    		                        
            1
                                    		                        
            0.5
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Plastiv cover
                                    		                        
            2
                                    		                        
            1
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Standard: 40ng/ml
                                    		                        
            1Vials （0.6ml）
                                    		                        
            0.5Vials （0.3ml）
                                    		                        
            See reagents preparation on page 3
                                    		        
                    
                                    
            Blank control
                                    		                        
            1Vials （1.0ml）
                                    		                        
            1Vials  （0.5ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Standard Diluent
                                    		                        
            1Vials （5ml）
                                    		                        
            1Vials   （2.5ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Biotinylated anti-PA
                                    		                        
            1Vials （6ml）
                                    		                        
            1Vials   （3.0ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Streptavidin-HRP
                                    		                        
            1Vials （10ml）
                                    		                        
            1Vials   （5.0ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Washing Buffer
                                    		                        
            1Vials （60ml）
                                    		                        
            1Vials   （30ml）
                                    		                        
            20× concentrate
                                    		        
                    
                                    
            Substrate  A
                                    		                        
            1Vials （6.0ml）
                                    		                        
            1Vials   （3.0ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Substrate  B
                                    		                        
            1Vials 6.0ml）
                                    		                        
            1Vials   （3.0ml）
                                    		                        
            Ready-to-use
                                    		        
                    
                                    
            Stopping Solution
                                    		                        
            1Vials （6.0ml）
                                    		                        
            1Vials   （3.0ml）
                                    		                        
            Ready-to-use
                                    		        
                
             
            MATERIAL REQUIRED BUT NOT PROVIDED
            l       Distilled water
            l       Pipettes:10ul、50ul、100ul、200ul、1000ul。
            l       Vortex mixer and magnetic stirrer.
             
             
            
							
							
							
						
            
					
            
				
            
			
            
		
            
		
		
		
		 



  
    
    				
			

		
    
    
    
    
    
    
    
    
    
    

    

     
     
     
	 




	 
	
	
	

            
	
            
		
            
			
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